Although animal models have limitations in terms of predictive and translational value to humans, they have played a major role in understanding this disease and anticancer drug discovery. Animal testing may provide safety benefits for new products, but... 2. Special molecules have evolved for iron acquisition, transport and storage in soluble, nontoxic forms. 1977. Animal models are a fundamental tool in the life sciences. Many of the items that are tested are never used.. "isLogged": "0", Disadvantages: Mice are different from humans, so not all results transfer directly to human responses. Query parameters: { Currently, most animal research is conducted on mice and rats. Generally, testing protocols are often painful to the animal test subjects, where they are deprived of food and water, force-fed, physically restrained in long periods, inflicted with pain and wounds to test for healing process effects and remedies, and even purposely killed as part of the process. Human diseases caused by genes that do not exist in zebrafish require a different animal model. I have been able to gather some of the leading experts in the field who will illustrate the many ways in which animal models can be used in studying wound healing and infection. Abstract: The genetic similarity between humans and nonhuman primates makes nonhuman primates uniquely suited as models for genetic research on complex physiological and behavioral phenotypes. They have advantages and disadvantages compared with other approaches. "openAccess": "0", Large animal species offer a variety of both natural and surrogate models, each with its advantages and disadvantages. 21 June 2013. Animal models are a fundamental tool in the life sciences. "isUnsiloEnabled": true, Limitations of Large-Animal Models. Genetically Modified Models The ob/ob Mouse. A serious assessment of the models used is necessary to draw conclusions and make decisions in an evidence-based manner. This article summarizes limitations typical of animal models. 1. The use of goats and sheep in research is much … This paper reviews the advantages and disadvantages of the experimental models commonly used as well as the methods that are more used in studies related to iron. In vitro studies, despite physiological differences to humans, are useful to increase knowledge related to this essential micronutrient. In this Taconic Insight, the focus will be on mice as a model used in drug discovery, yet many of the statements about mice are applicable to other species. Three depletion–-repletion techniques are mostly used: hemoglobin regeneration efficiency, relative biological values (RBV) and metabolic balance, which are official methods of the association of official analytical chemists. As discussed for other large animal models, these techniques are not as simple as the intraperitoneal and intravenous techniques available in small rodents. Isotopic techniques are the most recommended in studies related to iron, but their high cost and required logistic, making them difficult to use. The advantages and disadvantages of different categories of type 2 diabetic models are depicted in Table 2. Published online by Cambridge University Press:  In cancer research the most commonly used animal species are mice, rats, hamsters, rabbits, Guinea pigs, fish and amphibians. "shouldUseHypothesis": true, Some of the animals … 2016). However, the ability to manipulate both genetic and environmental factors in captive primate populations indicates the promise of genetic research with these important animal models … } "hasAccess": "0", Render date: 2021-01-23T09:34:52.109Z The mouse model, they say, remains an unsurpassed research tool, despite its imperfections. for this article. Ethical Considerations: The creation of transgenic mice is controversial and might ultimately increase the number of animals used in research because many mice must be bred Studies about the effects of iron on health are focused on iron metabolism or nutrition to prevent or treat iron deficiency and anemia. In a few instances, they represent the only reasonable approach. Iron metabolism is a complex process that includes interactions at the systemic level. In this review, we will discuss these advantages and disadvantages of commonly used laboratory animals including mouse, rat, rabbit, canine, swine, and sheep. If you should have access and can't see this content please, Iron, copper, and zinc absorption and turnover; the use of stable isotopes, Heme iron uptake by Caco-2 cells is a saturable, temperature sensitive and modulated by extracellular pH and potassium, Non heme iron absorption from a phytate rich meal is increased by the addition of small amounts of pork meat, Recent evidence from human and animal studies regarding iron status and infant development, Newly identified iron-binding protein in human duodenal mucosa, The iron exporter ferroportin/slc40a1 is essential for iron homeostasis, Nramp2 is mutated in the anemic Belgrade (b) rat: evidence of a role for Nramp2 in endosomal iron transport, Proceedings of the National Academy of Sciences of the United States of America, Assessment of iron absorption in mice by ICP-MS measurements of, Iron regulatory proteins are essential for intestinal function and control key iron absorption molecules in the duodenum, Iron uptake is enhanced in caco-2 cell monolayers by cysteine and reduced cysteinyl glycine, Iron uptake by isolated human enterocyte suspensions in vitro is dependent on body iron stores and inhibited by other metal cations, Behavioral consequences of developmental iron deficiency in infant rhesus monkeys, Nutritional iron supplementation studies based on enriched (57) Fe, added to milk in rats, and isotope pattern deconvolution-ICP-MS analysis, The enhancement of nonheme iron bioavailability by beef protein in the rat, Slc11a2 is required for intestinal iron absorption and erythropoiesis but dispensable in placenta and liver, The use of BeWo cells as an in vitro model for placental iron transport, American Journal of Physiology – Cell Physiology, Balancing acts: molecular control of mammalian iron metabolism, Ferritin-iron is released during boiling and in vitro gastric digestion, A mouse model of juvenile hemochromatosis, Effects of ascorbic acid, phytic acid and tannic acid on iron bioavailability from reconstituted ferritin measured by an in vitro digestion-Caco-2 cell model, Iron overloading and erythrophagocytosis increase ferroportin 1 (FPN1) expression in J774 macrophages, Evidence for a sequential transfer of iron amongst ferritin, transferrin and transferrin receptor during duodenal absorption of iron in rat and human, LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity, Non-transferrin iron reduction and uptake are regulated by transmembrane ascorbate cycling in k562 cells, Iron metabolism: microbes, mouse, and man, On the methods for studying the mechanism and bioavailability of iron, Soybean ferritin: implications for iron status of vegetarians, Iron absorption from soybean ferritin in non anemic woman, Iron and ascorbic acid: proposed fortification levels and recommended iron compounds, HIF-2alpha, but not HIF-1alpha, promotes iron absorption in mice, Worldwide prevalence of anemia in preschool aged children, pregnant women and non-pregnant women of reproductive age, Lack of the bone morphogenetic protein BMP6 induces massive iron overload, Effect of resistant and digestible starch on intestinal absorption of calcium, iron and zinc in infant pigs, Iron status and food matrix strongly affect the relative bioavailability of ferric pyrophosphate in humans, Mechanisms and regulation of intestinal iron absorption, Investigation of manganese and iron absorption by rat everted gut sac, Aluminium interference with iron absorption by everted gut sac, Journal of Islamic World Academic Science, Iron transport and homeostasis mechanisms: their role in health and disease, Iron deficiency anemia enhanced red blood cell oxidative stress, Proceedings of the National Academy of the Sciences USA, The gene encoding the iron regulatory peptide hepcidin is regulated by anemia, hypoxia, and inflammation, BMPER protein is a negative regulator of hepcidin and is up-regulated in hypotransferrinemic mice, The pig as an experimental model for elucidating the mechanisms governing dietary influence on mineral absorption, Iron bis-glycine chelate competes for the nonheme-iron absorption pathway, Identification of an intestinal folate transporter and the molecular basis for hereditary folate malabsorption, Bioavailability of heme iron of biscuit filling using piglets as an animal model for human, International Journal of Biological Sciences, Modulation of uptake of heme by rat small intestinal mucosa in iron deficiency, Iron-regulatory proteins limit hypoxia-inducible factor-2alpha expression in iron deficiency, Severity of neurodegeneration correlates with compromise of iron metabolism in mice with iron regulatory protein deficiencies, Annals of The New York Academy of Sciences, Supplementation with α-tocopherol or a combination of α-tocopherol and ascorbic acid protects the gastrointestinal tract of iron-deficient rats against iron-induced oxidative damage during iron repletion, Meat enhances nonheme iron absorption in pigs, Swine in biomedical research: management and models, Farm animals in biological research. 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